Fatty Acid Methyl Ester Profiles of the Genus Agrobacterium.

Accuracy, reproducibility, and interpretation of Fatty Acid methyl ester profiles of model bacterial communities.

We determined the accuracy and reproducibility of whole-community fatty acid methyl ester (FAME) analysis with two model bacterial communities differing in composition by using the Microbial ID, Inc. (MIDI), system. The biomass, taxonomic structure, and expected MIDI-FAME profiles under a variety of environmental conditions were known for these model communities a priori. Not all members of eac...

Identification and Characterization of Isolates of Phytophthora infestans Using Fatty Acid Methyl Ester (FAME) Profiles

The recent resurgence of the late blight pathogen, Phytophthora infestans (Mont.) de Bary, as a major agricultural threat, as well as the associated changes in genetic complexity of this organism, have necessitated careful scrutiny and characterization of populations of P. infestans so that appropriate control methods can be implemented. Several techniques for molecular and genetic characteriza...

FATTY ACID METHYL ESTER PROFILING OF Enterococcus and E

Automated determination of fatty acid methyl ester and cis/trans methyl ester composition of fats and oils.

The determination of the fatty acid composition (as methyl esters, FAMEs) of fats and oils and their cis/trans (CTME) distribution requires a simple, but manual and time-consuming sample preparation. The so-called BF3 method is often the preferred procedure. Because FAME/CTME analyses are encountered very frequently in the food industry, an automated, robot-based alternative is proposed which u...

Hydrogenation of fatty acid methyl ester to fatty alcohol at supercritical conditions

Hydrogenation of various types of oleochemicals is a major unit operation in industry. Natural fatty alcohols are important raw materials for surfactants and lubricants and can be produced by catalytic hydrogenation of fatty acid methyl esters (FAME). Commercial multiphase processes are necessarily operated at high pressures and high hydrogen-to-ester mole ration owing to low solubility of hydr...